- 2002: PhD in Synthetic Organic Chemistry, University of Bologna, IT
- 2002 – 2005: Postdoc, University College Dublin, IE
- 2006 – 2013: Lecturer, University College Dublin, IE
- 2014 – 2016: Senior Lecturer, University College Dublin, IE
- 2016 – 2019: Associate Professor in Biocatalysis and Enzyme Engineering, University of Nottingham, UK & Adjunct Professor, University College Dublin, IE
- since 2019: Professor of Pharmaceutical Chemistry, University of Bern, CH
We have a keen interest in the development of alternative biocatalysts for synthetic and industrial applications. Predominantly our research focuses on the discovery, characterization and evolution of enzymes originating from extremophilic organisms, more specifically salt adapted microbes which can withstand high salt concentrations. Currently we are working with a variety of enzymes encompassing oxido-reductases, transaminases, and hydrolases. The environmental adaptation of the organisms translates into unique enzymatic properties such as high tolerance to organic solvents, broad substrate scope, and remarkable stability.
To further extend enzymatic stability we have developed tailored immobilization strategies which allow us to use (and re-use) the enzymes in batch as well as in flow-systems. Flow-biocatalysis is currently our main tool in the synthesis of valuable molecules.
Our research is highly interdisciplinary with a number of collaborators in the US as well as Europe, and together we are looking at the assembly of semi-synthetic enzymes with enhanced biocatalytic properties.
We have a smaller synthetic stream in our lab which is dedicated to the incorporation of rigid, non-natural amino acids into peptidomimeticts which have demonstrated superior anti-cancer activity.
Publications recorded in BORIS (Bern Open Repository and Information System):
Contente, Martina Letizia; Farris, Stefano; Tamborini, Lucia; Molinari, Francesco; Paradisi, Francesca (2019). Flow-based enzymatic synthesis of melatonin and other high value tryptamine derivatives: a five-minute intensified process. Green Chemistry, 21(12), pp. 3263-3266. Royal Society of Chemistry 10.1039/C9GC01374A
Planchestainer, Matteo; Hegarty, Eimear; Heckmann, Christian M.; Gourlay, Louise J.; Paradisi, Francesca (2019). Widely applicable background depletion step enables transaminase evolution through solid-phase screening. Chemical Science, 10(23), pp. 5952-5958. The Royal Society of Chemistry 10.1039/C8SC05712E
Contente Martina Letizia, Contente; Paradisi, Francesca (2019). Transaminase-catalyzed continous synthesis of biogenic aldehydes (In Press). ChemBioChem Wiley-VCH 10.1002/cbic.201900356
Planchestainer, Matteo; Ségaud, Nathalie Sylvie Laetitia; Shanmugam, Muralidharan; McMaster, Jonathan; Paradisi, Francesca; Albrecht, Martin (2018). Carbene in Cupredoxin Protein Scaffolds: Replacement of a Histidine Ligand in the Active Site Substantially Alters Copper Redox Properties. Angewandte Chemie (International ed.), 57(33), pp. 10677-10682. Wiley-VCH 10.1002/anie.201807168
Conference or Workshop Item
Paradisi, Francesca (19 May 2019). Sustainable Catalysis: enzyme-mediated synthesis of high value chemicals and pharmaceuticals in Flow Reactors (Unpublished). In: 5th IMTB. Cavtat, Croatia.
- Member of the Royal Society of Chemistry
- Member of the International Society for Extremophiles
- Member of the Society for Applied Microbiology